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adar1  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc adar1
    Adar1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+adar1/ADAR1+XP+Rabbit+mAb/pm41800619-461-12-13
    Average 95 stars, based on 33 article reviews
    adar1 - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    other:

    Article Title: TSniffer : unbiased de novo identification of RNA editing sites and quantification of editing activity in RNA-seq data
    Article Snippet: Primary antibodies were rabbit anti-ADAR1 (clone D7E2M, Cell Signaling #14175) at 1:1,000; mouse anti-ADAR2 (clone 1.3.1, Millipore Sigma #MABE889) at 1:1,000; mouse anti-beta-actin − horseradish peroxidase (clone AC-15, Sigma-Aldrich #A3854) at 1:20,000.

    Article Title: TSniffer : Unbiased de novo identification of RNA editing sites and quantification of editing activity in RNA-seq data
    Article Snippet: Primary antibodies were rabbit anti-ADAR1 (clone D7E2M, Cell Signaling, #14175) at 1:1,000; mouse anti-ADAR2 (clone 1.3.1, Millipore Sigma, #MABE889) at 1:1,000; mouse anti-beta-actin−horseradish peroxidase (clone AC-15, Sigma-Aldrich, #A3854) at 1:20,000.

    Blocking Assay:

    Article Title: Z-Form Stabilization By The Zα Domain Of Adar1p150 Has Subtle Effects On A-To-I Editing
    Article Snippet: .. After blocking, they were incubated with either rabbit anti-ADAR1 (Cell Signaling, #14175) or rabbit anti-ADAR1p150 (Cell Signaling, #32136) primary antibodies at a 1:100 dilution in overnight at 4°C. .. The cells were then washed three times with 1X PBS for 5 minutes each and then incubated with Alexa Fluor 594 conjugated goat anti-rabbit secondary antibody (Cell Signaling, #8889) at a dilution of 1:500 in blocking buffer for 1 hour at room temperature.

    Incubation:

    Article Title: Z-Form Stabilization By The Zα Domain Of Adar1p150 Has Subtle Effects On A-To-I Editing
    Article Snippet: .. After blocking, they were incubated with either rabbit anti-ADAR1 (Cell Signaling, #14175) or rabbit anti-ADAR1p150 (Cell Signaling, #32136) primary antibodies at a 1:100 dilution in overnight at 4°C. .. The cells were then washed three times with 1X PBS for 5 minutes each and then incubated with Alexa Fluor 594 conjugated goat anti-rabbit secondary antibody (Cell Signaling, #8889) at a dilution of 1:500 in blocking buffer for 1 hour at room temperature.

    Article Title: Multimodal characterization of the responsiveness of eight hepatitis D virus genotype isolates to interferon-alpha treatment
    Article Snippet: .. Proteins were transferred onto nitrocellulose membranes by semidry transfer and incubated with primary antibodies as indicated below: rabbit anti-HDAg polyclonal antibody, mouse anti-β-actin (Sigma, A1978), mouse anti-GAPDH (Proteintech, 60004-1-lg), rabbit anti-ADAR1 (Cell Signaling, 14175), STAT1 monoclonal antibody (Proteintech, 66545-1-lg), STAT2 polyclonal antibody (Proteintech, 16674-1-AP), p-STAT1: phospho-STAT1 (Tyr701) polyclonal antibody (Proteintech, 28979-1-AP), and phospho-STAT2 (Tyr690) antibody (Affinity, AF3342). .. After overnight incubation at 4°C, membranes were washed with Tris-buffered saline with Tween-20 (TBST), and incubated with fluorescent-labeled secondary antibodies (LI-COR Biosciences) for 1 hour at room temperature, washed again, and imaged on a LI-COR Odyssey imaging system.

    Immunofluorescence:

    Article Title: An hepatitis B and D virus infection model using human pluripotent stem cell-derived hepatocytes
    Article Snippet: Mouse anti-CD63 (1:400 for immunofluorescence) , Santa Cruz Biotechnology , sc-5275. .. Rabbit anti-ADAR1 (1:1000 for immunofluorescence) , Cell Signaling , 14175. .. Rabbit anti-LAMP1 (1:200 for immunofluorescence; 1:1000 for western blot) , Cell Signaling , 9091.



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    a Structure model of a 158-base-long mini-construct that contains the long-range interaction LR1 base-pairing (mLR1). Mutations intended to disrupt the base-pairing from one side (mLR1A, green) or another side (mLR1B, orange) are indicated next to the original bases. Compensatory mutant mLR1AB contains both A and B mutations restoring the pairing of mLR1. b Top, PAGE showing proteins that were immunoprecipitated using biotinylated mLR1-WT RNA and negative control RNAs mLR1A (lane A), mLR1B (lane B) and Tetrahymena ribozyme (Teth). Input proteins from the whole cell lysate of Vero-E6 are also shown. Bottom, Western blot analysis of proteins from the pull-down assay using <t>anti-ADAR1</t> antibody. c EMSA experiment showing the binding of different concentrations of ADAR1 with indicated RNAs. d Quantification of the bound RNA fraction shown in c . Data are presented as mean ± SD ( n = 3 independent experiments). e A-to-I RNA editing level along SARS-CoV-2 WT, LR1A, LR1B and LR1AB mutant genomes in infected cells. N = 3 independent experiments (total 6 replicates). f Box-plot showing the distribution of editing level of each edited site. The total number of edited sites across three biological replicates is indicated. g Box-plot showing the distribution of the editing index ( n = 6) in samples as described for panel ( e ). h A-to-I RNA editing level along SARS-CoV-2 WT genome in cells transfected using a random ASO (ASO_CTRL), an ASO that targets a different region (ASO_1, against 1563-1582), or an ASO designed against the LR1 region (ASO_LR1). N = 2 independent experiments (total 4 replicates). i Box-plot showing the distribution of the editing level of each edited site. Total number of edited sites across two biological replicates is indicated. j Box-plot showing the editing index in samples treated as described for panel h ( n = 4). For box-plots, the box represents the 25–75th percentiles, median is indicated as the line. The whiskers show the minimum and maximum values. P -values were calculated using two-sided Wilcoxon Rank Sum Test. ns, not significant. Source data are provided as a Source Data file.
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    a Structure model of a 158-base-long mini-construct that contains the long-range interaction LR1 base-pairing (mLR1). Mutations intended to disrupt the base-pairing from one side (mLR1A, green) or another side (mLR1B, orange) are indicated next to the original bases. Compensatory mutant mLR1AB contains both A and B mutations restoring the pairing of mLR1. b Top, PAGE showing proteins that were immunoprecipitated using biotinylated mLR1-WT RNA and negative control RNAs mLR1A (lane A), mLR1B (lane B) and Tetrahymena ribozyme (Teth). Input proteins from the whole cell lysate of Vero-E6 are also shown. Bottom, Western blot analysis of proteins from the pull-down assay using <t>anti-ADAR1</t> antibody. c EMSA experiment showing the binding of different concentrations of ADAR1 with indicated RNAs. d Quantification of the bound RNA fraction shown in c . Data are presented as mean ± SD ( n = 3 independent experiments). e A-to-I RNA editing level along SARS-CoV-2 WT, LR1A, LR1B and LR1AB mutant genomes in infected cells. N = 3 independent experiments (total 6 replicates). f Box-plot showing the distribution of editing level of each edited site. The total number of edited sites across three biological replicates is indicated. g Box-plot showing the distribution of the editing index ( n = 6) in samples as described for panel ( e ). h A-to-I RNA editing level along SARS-CoV-2 WT genome in cells transfected using a random ASO (ASO_CTRL), an ASO that targets a different region (ASO_1, against 1563-1582), or an ASO designed against the LR1 region (ASO_LR1). N = 2 independent experiments (total 4 replicates). i Box-plot showing the distribution of the editing level of each edited site. Total number of edited sites across two biological replicates is indicated. j Box-plot showing the editing index in samples treated as described for panel h ( n = 4). For box-plots, the box represents the 25–75th percentiles, median is indicated as the line. The whiskers show the minimum and maximum values. P -values were calculated using two-sided Wilcoxon Rank Sum Test. ns, not significant. Source data are provided as a Source Data file.
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    Image Search Results


    a Structure model of a 158-base-long mini-construct that contains the long-range interaction LR1 base-pairing (mLR1). Mutations intended to disrupt the base-pairing from one side (mLR1A, green) or another side (mLR1B, orange) are indicated next to the original bases. Compensatory mutant mLR1AB contains both A and B mutations restoring the pairing of mLR1. b Top, PAGE showing proteins that were immunoprecipitated using biotinylated mLR1-WT RNA and negative control RNAs mLR1A (lane A), mLR1B (lane B) and Tetrahymena ribozyme (Teth). Input proteins from the whole cell lysate of Vero-E6 are also shown. Bottom, Western blot analysis of proteins from the pull-down assay using anti-ADAR1 antibody. c EMSA experiment showing the binding of different concentrations of ADAR1 with indicated RNAs. d Quantification of the bound RNA fraction shown in c . Data are presented as mean ± SD ( n = 3 independent experiments). e A-to-I RNA editing level along SARS-CoV-2 WT, LR1A, LR1B and LR1AB mutant genomes in infected cells. N = 3 independent experiments (total 6 replicates). f Box-plot showing the distribution of editing level of each edited site. The total number of edited sites across three biological replicates is indicated. g Box-plot showing the distribution of the editing index ( n = 6) in samples as described for panel ( e ). h A-to-I RNA editing level along SARS-CoV-2 WT genome in cells transfected using a random ASO (ASO_CTRL), an ASO that targets a different region (ASO_1, against 1563-1582), or an ASO designed against the LR1 region (ASO_LR1). N = 2 independent experiments (total 4 replicates). i Box-plot showing the distribution of the editing level of each edited site. Total number of edited sites across two biological replicates is indicated. j Box-plot showing the editing index in samples treated as described for panel h ( n = 4). For box-plots, the box represents the 25–75th percentiles, median is indicated as the line. The whiskers show the minimum and maximum values. P -values were calculated using two-sided Wilcoxon Rank Sum Test. ns, not significant. Source data are provided as a Source Data file.

    Journal: Nature Communications

    Article Title: A conserved long-range RNA interaction in SARS-CoV-2 recruits ADAR1 to enhance virus proliferation

    doi: 10.1038/s41467-025-63297-2

    Figure Lengend Snippet: a Structure model of a 158-base-long mini-construct that contains the long-range interaction LR1 base-pairing (mLR1). Mutations intended to disrupt the base-pairing from one side (mLR1A, green) or another side (mLR1B, orange) are indicated next to the original bases. Compensatory mutant mLR1AB contains both A and B mutations restoring the pairing of mLR1. b Top, PAGE showing proteins that were immunoprecipitated using biotinylated mLR1-WT RNA and negative control RNAs mLR1A (lane A), mLR1B (lane B) and Tetrahymena ribozyme (Teth). Input proteins from the whole cell lysate of Vero-E6 are also shown. Bottom, Western blot analysis of proteins from the pull-down assay using anti-ADAR1 antibody. c EMSA experiment showing the binding of different concentrations of ADAR1 with indicated RNAs. d Quantification of the bound RNA fraction shown in c . Data are presented as mean ± SD ( n = 3 independent experiments). e A-to-I RNA editing level along SARS-CoV-2 WT, LR1A, LR1B and LR1AB mutant genomes in infected cells. N = 3 independent experiments (total 6 replicates). f Box-plot showing the distribution of editing level of each edited site. The total number of edited sites across three biological replicates is indicated. g Box-plot showing the distribution of the editing index ( n = 6) in samples as described for panel ( e ). h A-to-I RNA editing level along SARS-CoV-2 WT genome in cells transfected using a random ASO (ASO_CTRL), an ASO that targets a different region (ASO_1, against 1563-1582), or an ASO designed against the LR1 region (ASO_LR1). N = 2 independent experiments (total 4 replicates). i Box-plot showing the distribution of the editing level of each edited site. Total number of edited sites across two biological replicates is indicated. j Box-plot showing the editing index in samples treated as described for panel h ( n = 4). For box-plots, the box represents the 25–75th percentiles, median is indicated as the line. The whiskers show the minimum and maximum values. P -values were calculated using two-sided Wilcoxon Rank Sum Test. ns, not significant. Source data are provided as a Source Data file.

    Article Snippet: Western blot analysis was performed according to standard procedures using a 1:1000 dilution of anti-ADAR1 (E6X9R) XP(R) rabbit monoclonal antibody (Cell Signalling Technology) and a 1:5000 dilution of secondary anti-rabbit antibody.

    Techniques: Construct, Mutagenesis, Immunoprecipitation, Negative Control, Western Blot, Pull Down Assay, Binding Assay, Infection, Transfection